Tolonium chloride
IUPAC name	(7-amino-8-methyl- phenothiazin-3-ylidene)- dimethyl-ammonium
Other names	Toluidine blue O
Identifiers
CAS number	92-31-9
PubChem	7084
MeSH	Tolonium+chloride
SMILES	CC1=CC2=C(C=C1N)SC3= CC(=[N+](C)C)C=CC3=N2
Properties
Molecular formula	C15H16N3S+
Molar mass	270.374 g/mol
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Tolonium chloride (INN, also known as toluidine blue O) is a compound used in histology.

[edit] Test for lignin

Toluidine blue solution is used in testing for lignin, a complex organic molecule that bonds to cellulose fibres and strengthens and hardens the cell walls in plants. A positive toluidine blue test causes the solution to turn from blue to blue-green. A similar test can be performed with phloroglucinol-HCl solution, which turns red.

[edit] Other histological uses

Toluidine Blue is often used to identify Mast cells. It is also used to stain proteoglycans and glycosaminoglycans in tissues such as cartilage.

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