Twi is a language spoken in Ghana.

Twi may also refer to:

• Twi-, a numerical prefix
• Twi ba, a percussive musical instrument
• Twi'lek, an alien species in the Star Wars universe

TWI as an acronym may refer to:

• The Way International, a religious organization considered by some to be a cult
• The Welding Institute, a research and technology organisation based in the UK
• Trade weighted index, an economic instrument used to compare exchange rates
• Training Within Industry, a service in the US that provided consultancy to war-related industries during World War II
• Trans World International, a distributor and producer of televised sports
• TWI-Bus or Two Wire Interface, a variant of I²C
• Topographic Wetness Index, an algorithm based on slope and flow accumulation. Otherwise known as Compound Topographic Index
• Two-Way Immersion is often used interchangeably with the term Dual language, which is a form of education in which students are taught literacy and content in two languages.
• Tread Wear Indicator
• Tolerable Weekly Intake

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