| Sulforhodamine B |
 |
| IUPAC name | 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate |
| Identifiers |
| CAS number | 3520-42-1 Y |
| PubChem | 65191 |
| EC number | 220-025-2 |
| SMILES | O=S(=O)(O)c4ccc(C=1c3c(OC=2C=1\C=C/C(=[N+](/CC)CC)/C=2)cc(cc3)N(CC)CC)c(c4)S([O-])(=O)=O |
| InChI | 1/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35) |
| InChI key | IOOMXAQUNPWDLL-UHFFFAOYAJ |
| Properties |
| Molecular formula | C27H30N2O7S2 |
| Molar mass | 558.666 g/mol |
Y (what is this?) (verify) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
| Infobox references |
Sulforhodamine B or kiton red (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. The red, solid, water-soluble dye is primarily used as a polar tracer.
The dye has maximal absorbance at 585 nm light and maximal fluorescence emission at 607 nm light. It does not exhibit pH dependent absorption or fluorescence over the range of 3 to 10.[1]
[edit] References
- ^ Coppeta, Rogers (1998). "Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements". Experiments in Fluids 25: 1–15;. doi:10.1007/s003480050202.