Sephadex is a trademark for cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin.^[1][2] The name is an anagram of separation Pharmacia dextran. It is normally manufactured in a bead form and most commonly used for gel filtration columns. By varying the degree of cross-linking, the fractionation properties of the gel can be altered.

These highly specialized gel filtration and chromatographic media are composed of macroscopic beads synthetically derived from the polysaccharide, dextran. The organic chains are cross-linked to give a three dimensional network having functional ionic groups attached by ether linkages to glucose units of the polysaccharide chains.

Available forms include anion and cation exchangers, as well as gelfiltration resins, with varying degrees of porosity; bead sizes fall in discrete ranges between 20 and 300 µm.

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[edit] See also

• PEGylation
• Size exclusion chromatography