Phi X 174 Information & Phi X 174 Links at HealthHaven.com

Phi X 174
Virus classification
Group:	Group II (ssDNA)
Family:	Microviridae;
Genus:	Microvirus;
Species:	phi X 174 phage;

Structure of phage phi X 174 capsid

The phi X 174 (or phi X) bacteriophage was the first DNA-based genome to be sequenced. This work was completed by Fred Sanger and his team in 1977.^[1] In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.^[2]

This phage has a very small amount of DNA. Phi X has 11 genes in 5386 bases (it is single stranded) in a circular topology; several of these genes express similar function in two groups. The GC-content is 44% and 95% of nucleotides are coding genes.

In 2003, it was reported that the whole genome of phi X 174 had been assembled synthetically from scratch.^[3]

[edit] See also

Bacteriophage MS2

[edit] References

^ Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M (1977-02-24). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature 265 (5596): 687–695.
^ Fiers W, Sinsheimer RL (1962). "The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure". J. Mol. Biol. 5: 424–434.
^ Smith, Hamilton O.; Clyde A. Hutchison, Cynthia Pfannkoch, J. Craig Venter (2003-12-23). "Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences 100 (26): 15440–15445. doi:10.1073/pnas.2237126100. http://www.pnas.org/cgi/content/abstract/100/26/15440. Retrieved 2007-10-08.

[edit] External links

Description & Restriction Map: PhiX174 DNA

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[0] Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M (1977-02-24). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature 265 (5596): 687–695.

[1] Fiers W, Sinsheimer RL (1962). "The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure". J. Mol. Biol. 5: 424–434.

[2] Smith, Hamilton O.; Clyde A. Hutchison, Cynthia Pfannkoch, J. Craig Venter (2003-12-23). "Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences 100 (26): 15440–15445. doi:10.1073/pnas.2237126100. http://www.pnas.org/cgi/content/abstract/100/26/15440. Retrieved 2007-10-08.

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