PPP2CA Information & PPP2CA Links at HealthHaven.com

Protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
	; PDB rendering based on 2iae.
Available structures
	2iae, 2ie3, 2ie4, 2npp, 2nyl, 2nym
Identifiers
Symbols	PPP2CA; PP2CA; PP2Ac; RP-C
External IDs	OMIM: 176915 MGI: 1321159 HomoloGene: 37660 GeneCards: PPP2CA Gene
Gene ontology
Molecular function	• phosphoprotein phosphatase activity; • iron ion binding; • protein binding; • hydrolase activity; • manganese ion binding; • metal ion binding; • protein heterodimerization activity;
Cellular component	• protein phosphatase type 2A complex; • soluble fraction; • nucleus; • cytoplasm; • mitochondrion; • cytosol; • plasma membrane; • microtubule cytoskeleton;
Biological process	• regulation of progression through cell cycle; • inactivation of MAPK activity; • regulation of DNA replication; • protein amino acid dephosphorylation; • ceramide metabolic process; • induction of apoptosis; • mesoderm development; • RNA splicing; • response to organic substance; • second-messenger-mediated signaling; • regulation of Wnt receptor signaling pathway; • regulation of cell adhesion; • negative regulation of cell growth; • regulation of growth; • negative regulation of tyrosine phosphorylation of Stat3 protein; • regulation of transcription; • regulation of cell differentiation;
RNA expression pattern
	More reference expression data
Orthologs

edit

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform is an enzyme that in humans is encoded by the PPP2CA gene.^[1]

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.^[2]

[edit] Interactions

PPP2CA has been shown to interact with CCNG2,^[3] CTTNBP2,^[4] MOBKL3,^[4] Bestrophin 1,^[5] TLX1,^[6] Bcl-2,^[7] PPP2R3B,^[4]^[8] Cyclin-dependent kinase 2,^[9] STRN3,^[4] Cyclin-dependent kinase 6,^[9] IGBP1,^[4]^[10]^[11]^[12] PPP2R5E,^[4]^[13] STRN,^[4] PPP2R5D,^[4]^[13] PPP2R2A,^[4]^[14] PPP2R1B,^[4]^[15] PPP2R1A,^[4]^[15] CTTNBP2NL,^[4] FAM40A,^[4] PPP2R5C,^[4]^[13]^[16] PPP2R5B^[4]^[13] and PPP2R5A.^[4]^[13]

[edit] See also

PPP2CB

[edit] References

^ Jones TA, Barker HM, da Cruz e Silva EF, Mayer-Jaekel RE, Hemmings BA, Spurr NK, Sheer D, Cohen PT (Apr 1993). "Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23→q31 and 8p12→p11.2, respectively". Cytogenet Cell Genet 63 (1): 35–41. PMID 8383590.
^ "Entrez Gene: PPP2CA protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5515.
^ Bennin, David A; Don Aruni S Arachchige, Brake Tiffany, McKenzie Jennifer L, Rosenbaum Heidi, Ortiz Linette, DePaoli-Roach Anna A, Horne Mary C (Jul. 2002). "Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest". J. Biol. Chem. (United States) 277 (30): 27449–67. doi:10.1074/jbc.M111693200. ISSN 0021-9258. PMID 11956189.
^ ^a ^b ^c ^d ^e ^f ^g ^h ⁱ ^j ^k ^l ^m ⁿ ^o ^p Goudreault, Marilyn; D'Ambrosio Lisa M, Kean Michelle J, Mullin Michael J, Larsen Brett G, Sanchez Amy, Chaudhry Sidharth, Chen Ginny I, Sicheri Frank, Nesvizhskii Alexey I, Aebersold Ruedi, Raught Brian, Gingras Anne-Claude (Jan. 2009). "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein". Mol. Cell Proteomics (United States) 8 (1): 157–71. doi:10.1074/mcp.M800266-MCP200. PMID 18782753.
^ Marmorstein, Lihua Y; McLaughlin Precious J, Stanton J Brett, Yan Lin, Crabb John W, Marmorstein Alan D (Aug. 2002). "Bestrophin interacts physically and functionally with protein phosphatase 2A". J. Biol. Chem. (United States) 277 (34): 30591–7. doi:10.1074/jbc.M204269200. ISSN 0021-9258. PMID 12058047.
^ Kawabe, T; Muslin A J, Korsmeyer S J (Jan. 1997). "HOX11 interacts with protein phosphatases PP2A and PP1 and disrupts a G2/M cell-cycle checkpoint". Nature (ENGLAND) 385 (6615): 454–8. doi:10.1038/385454a0. ISSN 0028-0836. PMID 9009195.
^ Deng, X; Ito T, Carr B, Mumby M, May W S (Dec. 1998). "Reversible phosphorylation of Bcl2 following interleukin 3 or bryostatin 1 is mediated by direct interaction with protein phosphatase 2A". J. Biol. Chem. (UNITED STATES) 273 (51): 34157–63. ISSN 0021-9258. PMID 9852076.
^ Yan, Z; Fedorov S A, Mumby M C, Williams R S (Feb. 2000). "PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells". Mol. Cell. Biol. (UNITED STATES) 20 (3): 1021–9. ISSN 0270-7306. PMID 10629059.
^ ^a ^b Cheng, A; Kaldis P, Solomon M J (Nov. 2000). "Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2C alpha and beta 2 isoforms". J. Biol. Chem. (UNITED STATES) 275 (44): 34744–9. doi:10.1074/jbc.M006210200. ISSN 0021-9258. PMID 10934208.
^ Gingras, Anne-Claude; Caballero Michael, Zarske Marcel, Sanchez Amy, Hazbun Tony R, Fields Stanley, Sonenberg Nahum, Hafen Ernst, Raught Brian, Aebersold Ruedi (Nov. 2005). "A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity". Mol. Cell Proteomics (United States) 4 (11): 1725–40. doi:10.1074/mcp.M500231-MCP200. ISSN 1535-9476. PMID 16085932.
^ Chen, J; Peterson R T, Schreiber S L (Jun. 1998). "Alpha 4 associates with protein phosphatases 2A, 4, and 6". Biochem. Biophys. Res. Commun. (UNITED STATES) 247 (3): 827–32. doi:10.1006/bbrc.1998.8792. ISSN 0006-291X. PMID 9647778.
^ Chung, H; Nairn A C, Murata K, Brautigan D L (Aug. 1999). "Mutation of Tyr307 and Leu309 in the protein phosphatase 2A catalytic subunit favors association with the alpha 4 subunit which promotes dephosphorylation of elongation factor-2". Biochemistry (UNITED STATES) 38 (32): 10371–6. doi:10.1021/bi990902g. ISSN 0006-2960. PMID 10441131.
^ ^a ^b ^c ^d ^e McCright, B; Rivers A M, Audlin S, Virshup D M (Sep. 1996). "The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm". J. Biol. Chem. (UNITED STATES) 271 (36): 22081–9. ISSN 0021-9258. PMID 8703017.
^ Kamibayashi, C; Lickteig R L, Estes R, Walter G, Mumby M C (Oct. 1992). "Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits". J. Biol. Chem. (UNITED STATES) 267 (30): 21864–72. ISSN 0021-9258. PMID 1328247.
^ ^a ^b Zhou, Jin; Pham Huong T, Ruediger Ralf, Walter Gernot (Jan. 2003). "Characterization of the Aalpha and Abeta subunit isoforms of protein phosphatase 2A: differences in expression, subunit interaction, and evolution". Biochem. J. (England) 369 (Pt 2): 387–98. doi:10.1042/BJ20021244. ISSN 0264-6021. PMID 12370081.
^ Ito, A; Kataoka T R, Watanabe M, Nishiyama K, Mazaki Y, Sabe H, Kitamura Y, Nojima H (Feb. 2000). "A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation". EMBO J. (ENGLAND) 19 (4): 562–71. doi:10.1093/emboj/19.4.562. ISSN 0261-4189. PMID 10675325.

[edit] Further reading

Cohen P, Cohen PT (1990). "Protein phosphatases come of age.". J. Biol. Chem. 264 (36): 21435–8. PMID 2557326.
Zolnierowicz S (2000). "Type 2A protein phosphatase, the complex regulator of numerous signaling pathways.". Biochem. Pharmacol. 60 (8): 1225–35. doi:10.1016/S0006-2952(00)00424-X. PMID 11007961.
Kamibayashi C, Lickteig RL, Estes R, et al. (1992). "Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits.". J. Biol. Chem. 267 (30): 21864–72. PMID 1328247.
Goedert M, Cohen ES, Jakes R, Cohen P (1992). "p42 MAP kinase phosphorylation sites in microtubule-associated protein tau are dephosphorylated by protein phosphatase 2A1. Implications for Alzheimer's disease [corrected]". FEBS Lett. 312 (1): 95–9. doi:10.1016/0014-5793(92)81418-L. PMID 1330687.
Khew-Goodall Y, Mayer RE, Maurer F, et al. (1991). "Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genes.". Biochemistry 30 (1): 89–97. doi:10.1021/bi00215a014. PMID 1846293.
Scheidtmann KH, Virshup DM, Kelly TJ (1991). "Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity.". J. Virol. 65 (4): 2098–101. PMID 1848320.
Scheidtmann KH, Mumby MC, Rundell K, Walter G (1991). "Dephosphorylation of simian virus 40 large-T antigen and p53 protein by protein phosphatase 2A: inhibition by small-t antigen.". Mol. Cell. Biol. 11 (4): 1996–2003. PMID 1848668.
Virshup DM, Kauffman MG, Kelly TJ (1990). "Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A.". Embo J. 8 (12): 3891–8. PMID 2555176.
Arino J, Woon CW, Brautigan DL, et al. (1988). "Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes.". Proc. Natl. Acad. Sci. U.S.A. 85 (12): 4252–6. doi:10.1073/pnas.85.12.4252. PMID 2837763.
Stone SR, Mayer R, Wernet W, et al. (1989). "The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A alpha catalytic subunit.". Nucleic Acids Res. 16 (23): 11365. doi:10.1093/nar/16.23.11365. PMID 2849764.
Carrey EA, Campbell DG, Hardie DG (1986). "Phosphorylation and activation of hamster carbamyl phosphate synthetase II by cAMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis.". Embo J. 4 (13B): 3735–42. PMID 4092695.
McCright B, Virshup DM (1995). "Identification of a new family of protein phosphatase 2A regulatory subunits.". J. Biol. Chem. 270 (44): 26123–8. doi:10.1074/jbc.270.44.26123. PMID 7592815.
Favre B, Zolnierowicz S, Turowski P, Hemmings BA (1994). "The catalytic subunit of protein phosphatase 2A is carboxyl-methylated in vivo.". J. Biol. Chem. 269 (23): 16311–7. PMID 8206937.
Redpath NT, Price NT, Severinov KV, Proud CG (1993). "Regulation of elongation factor-2 by multisite phosphorylation.". Eur. J. Biochem. 213 (2): 689–99. doi:10.1111/j.1432-1033.1993.tb17809.x. PMID 8386634.
Muramatsu T, Kincaid RL (1993). "Molecular cloning of a full-length cDNA encoding the catalytic subunit of human calmodulin-dependent protein phosphatase (calcineurin A alpha).". Biochim. Biophys. Acta 1178 (1): 117–20. doi:10.1016/0167-4889(93)90117-8. PMID 8392375.
Kissinger CR, Parge HE, Knighton DR, et al. (1996). "Crystal structures of human calcineurin and the human FKBP12-FK506-calcineurin complex.". Nature 378 (6557): 641–4. doi:10.1038/378641a0. PMID 8524402.
Ohguro H, Rudnicka-Nawrot M, Buczyłko J, et al. (1996). "Structural and enzymatic aspects of rhodopsin phosphorylation.". J. Biol. Chem. 271 (9): 5215–24. doi:10.1074/jbc.271.9.5215. PMID 8617805.
McCright B, Rivers AM, Audlin S, Virshup DM (1996). "The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.". J. Biol. Chem. 271 (36): 22081–9. doi:10.1074/jbc.271.36.22081. PMID 8703017.

This article on a gene on chromosome 5 is a stub. You can help Wikipedia by expanding it.

[pmid8383590-0] Jones TA, Barker HM, da Cruz e Silva EF, Mayer-Jaekel RE, Hemmings BA, Spurr NK, Sheer D, Cohen PT (Apr 1993). "Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23→q31 and 8p12→p11.2, respectively". Cytogenet Cell Genet 63 (1): 35–41. PMID 8383590.

[1] "Entrez Gene: PPP2CA protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5515.

[pmid11956189-2] Bennin, David A; Don Aruni S Arachchige, Brake Tiffany, McKenzie Jennifer L, Rosenbaum Heidi, Ortiz Linette, DePaoli-Roach Anna A, Horne Mary C (Jul. 2002). "Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest". J. Biol. Chem. (United States) 277 (30): 27449–67. doi:10.1074/jbc.M111693200. ISSN 0021-9258. PMID 11956189.

[pmid18782753-3] ^ ^a ^b ^c ^d ^e ^f ^g ^h ⁱ ^j ^k ^l ^m ⁿ ^o ^p Goudreault, Marilyn; D'Ambrosio Lisa M, Kean Michelle J, Mullin Michael J, Larsen Brett G, Sanchez Amy, Chaudhry Sidharth, Chen Ginny I, Sicheri Frank, Nesvizhskii Alexey I, Aebersold Ruedi, Raught Brian, Gingras Anne-Claude (Jan. 2009). "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein". Mol. Cell Proteomics (United States) 8 (1): 157–71. doi:10.1074/mcp.M800266-MCP200. PMID 18782753.

[pmid12058047-4] Marmorstein, Lihua Y; McLaughlin Precious J, Stanton J Brett, Yan Lin, Crabb John W, Marmorstein Alan D (Aug. 2002). "Bestrophin interacts physically and functionally with protein phosphatase 2A". J. Biol. Chem. (United States) 277 (34): 30591–7. doi:10.1074/jbc.M204269200. ISSN 0021-9258. PMID 12058047.

[pmid9009195-5] Kawabe, T; Muslin A J, Korsmeyer S J (Jan. 1997). "HOX11 interacts with protein phosphatases PP2A and PP1 and disrupts a G2/M cell-cycle checkpoint". Nature (ENGLAND) 385 (6615): 454–8. doi:10.1038/385454a0. ISSN 0028-0836. PMID 9009195.

[pmid9852076-6] Deng, X; Ito T, Carr B, Mumby M, May W S (Dec. 1998). "Reversible phosphorylation of Bcl2 following interleukin 3 or bryostatin 1 is mediated by direct interaction with protein phosphatase 2A". J. Biol. Chem. (UNITED STATES) 273 (51): 34157–63. ISSN 0021-9258. PMID 9852076.

[pmid10629059-7] Yan, Z; Fedorov S A, Mumby M C, Williams R S (Feb. 2000). "PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells". Mol. Cell. Biol. (UNITED STATES) 20 (3): 1021–9. ISSN 0270-7306. PMID 10629059.

[pmid10934208-8] Cheng, A; Kaldis P, Solomon M J (Nov. 2000). "Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2C alpha and beta 2 isoforms". J. Biol. Chem. (UNITED STATES) 275 (44): 34744–9. doi:10.1074/jbc.M006210200. ISSN 0021-9258. PMID 10934208.

[pmid16085932-9] Gingras, Anne-Claude; Caballero Michael, Zarske Marcel, Sanchez Amy, Hazbun Tony R, Fields Stanley, Sonenberg Nahum, Hafen Ernst, Raught Brian, Aebersold Ruedi (Nov. 2005). "A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity". Mol. Cell Proteomics (United States) 4 (11): 1725–40. doi:10.1074/mcp.M500231-MCP200. ISSN 1535-9476. PMID 16085932.

[pmid9647778-10] Chen, J; Peterson R T, Schreiber S L (Jun. 1998). "Alpha 4 associates with protein phosphatases 2A, 4, and 6". Biochem. Biophys. Res. Commun. (UNITED STATES) 247 (3): 827–32. doi:10.1006/bbrc.1998.8792. ISSN 0006-291X. PMID 9647778.

[pmid10441131-11] Chung, H; Nairn A C, Murata K, Brautigan D L (Aug. 1999). "Mutation of Tyr307 and Leu309 in the protein phosphatase 2A catalytic subunit favors association with the alpha 4 subunit which promotes dephosphorylation of elongation factor-2". Biochemistry (UNITED STATES) 38 (32): 10371–6. doi:10.1021/bi990902g. ISSN 0006-2960. PMID 10441131.

[pmid8703017-12] McCright, B; Rivers A M, Audlin S, Virshup D M (Sep. 1996). "The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm". J. Biol. Chem. (UNITED STATES) 271 (36): 22081–9. ISSN 0021-9258. PMID 8703017.

[pmid1328247-13] Kamibayashi, C; Lickteig R L, Estes R, Walter G, Mumby M C (Oct. 1992). "Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits". J. Biol. Chem. (UNITED STATES) 267 (30): 21864–72. ISSN 0021-9258. PMID 1328247.

[pmid12370081-14] Zhou, Jin; Pham Huong T, Ruediger Ralf, Walter Gernot (Jan. 2003). "Characterization of the Aalpha and Abeta subunit isoforms of protein phosphatase 2A: differences in expression, subunit interaction, and evolution". Biochem. J. (England) 369 (Pt 2): 387–98. doi:10.1042/BJ20021244. ISSN 0264-6021. PMID 12370081.

[pmid10675325-15] Ito, A; Kataoka T R, Watanabe M, Nishiyama K, Mazaki Y, Sabe H, Kitamura Y, Nojima H (Feb. 2000). "A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation". EMBO J. (ENGLAND) 19 (4): 562–71. doi:10.1093/emboj/19.4.562. ISSN 0261-4189. PMID 10675325.

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Contents

[edit] Interactions

[edit] See also

[edit] References

[edit] Further reading