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Aerobic and anaerobic bacteria can be identified by growing them in liquid culture:
1. Obligate aerobe
2. Obligate anaerobe
3. Facultative anaerobic organism (continuum with "Facultative aerobic organism")
4. Microaerophile
5. Aerotolerant

An Obligate aerobe is an aerobic organism that requires oxygen to grow.

Examples of Obligate Aerobic Bacteria: Nocardia (Gram-positive), Pseudomonas aeruginosa (Gram-negative), Mycobacterium tuberculosis (acid-fast), and Bacillus (Gram-positive).

Obligate Aerobes An organism that requires oxygen for cellular respiration and cannot live without it. Through Cellular Respiration, these organisms use oxygen to oxidize substrates( i.e. Sugars and fats) in order to obtain energy They use oxygen as the terminal electron acceptor during aerobic respiration Have the advantage of yielding more energy than obligate anaerobes. Face high levels of oxidative stress( a highly oxidized environment w/n cells that is thought to promote HIV replication because cells are forced into a highly activated state due to loss of control of their regulatory systems)[[1]]