A locule (pl. loculi; from Latin loculus, meaning "little place") is a small cavity or compartment within an organ or part of an organism (animal, plant or fungus).

In plants, the term locule usually refers to a chamber within an ovary (gynoecium or carpel of the flower and fruits). Depending on the number of locules in the ovary, carpels and fruits can be classified as uni-locular, bi-locular or multi-locular. The locules contain the ovules or seeds. The term may also refer to chambers within anthers containing pollen.

In the Loculoascomycetes, a group of sac fungi, locules are chambers containing perithecia with asci and ascospores.

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