| Autonomous community | University | Seat of the Rectorate |
| Andalusia | Public | International University of Andalusia | Seville |
| Pablo de Olavide University | Seville |
| University of Almería | Almería |
| University of Cádiz | Cádiz |
| University of Córdoba | Córdoba |
| University of Granada | Granada |
| University of Huelva | Huelva |
| University of Jaén | Jaén |
| University of Málaga | Málaga |
| University of Seville | Seville |
| Private | Ferdinand III University | Seville |
| Aragon | Public | University of Zaragoza | Zaragoza |
| Private | Saint George University | Villanueva de Gállego, Zaragoza |
| Asturias | Public | University of Oviedo | Oviedo |
| Balearic Islands | Public | University of the Balearic Islands | Palma |
| Basque Country | Public | University of the Basque Country | Leioa, Biscay |
| Private | University of Deusto | Bilbao |
| University of Mondragón | Mondragón, Guipúzcoa |
| Canary Islands | Public | University of La Laguna | San Cristóbal de La Laguna, Santa Cruz de Tenerife |
| University of Las Palmas de Gran Canaria | Las Palmas de Gran Canaria |
| Cantabria | Public | University of Cantabria | Santander |
| Castile-La Mancha | Public | University of Castile-La Mancha | Ciudad Real |
| Castile and León | Public | University of Burgos | Burgos |
| University of León | León |
| University of Salamanca | Salamanca |
| University of Valladolid | Valladolid |
| Private | Catholic University of Ávila | Ávila |
| IE University | Segovia |
| Miguel de Cervantes European University | Valladolid |
| Pontifical University of Salamanca | Salamanca;
out of Castile and León, also in Madrid |
| Catalonia | Public | Autonomous University of Barcelona | Cerdanyola del Vallès, Barcelona |
| Polytechnic University of Catalonia | Barcelona |
| Pompeu Fabra University | Barcelona |
| Rovira i Virgili University | Tarragona |
| University of Barcelona | Barcelona |
| University of Girona | Girona |
| University of Lleida | Lleida |
| Private | Abat Oliba CEU University | Barcelona |
| International University of Catalonia | Barcelona |
| Open University of Catalonia | Barcelona |
| Ramon Llull University | Barcelona |
| University of Vic | Vic, Barcelona |
| Extremadura | Public | University of Extremadura | Badajoz |
| Galicia | Public | University of A Coruña | A Coruña |
| University of Santiago de Compostela | Santiago de Compostela |
| University of Vigo | Vigo, Pontevedra |
| La Rioja | Public | University of La Rioja | Logroño |
| Madrid | Public | Autonomous University of Madrid | Madrid |
| Charles III University of Madrid | Getafe |
| Complutense University of Madrid | Madrid |
| King John Charles University | Móstoles |
| Menéndez Pelayo International University | Madrid;
out of Madrid, also in A Coruña; Barcelona; Cuenca; Granada; La Línea de la Concepción, in Cádiz; Sallent de Gállego, in Huesca; Santa Cruz de Tenerife; Santander; Seville; and Valencia |
| National University of Distance Education | Madrid |
| Polytechnic University of Madrid | Madrid |
| University of Alcalá | Alcalá de Henares;
out of Madrid, also in Guadalajara |
| Private[1] | Universidad a Distancia de Madrid | Madrid |
| Alfonso X University | Villanueva de la Cañada |
| Antonio de Nebrija University | Hoyo de Manzanares |
| Camilo José Cela University | Villanueva de la Cañada |
| Universidad CEU San Pablo | Madrid |
| European University of Madrid | Villaviciosa de Odón |
| Francisco de Vitoria University | Pozuelo de Alarcón |
| Pontifical University of Comillas | Madrid |
| Murcia | Public | University of Murcia | Murcia |
| Universidad Politécnica de Cartagena | Cartagena |
| Private | Universidad Católica San Antonio de Murcia | Murcia |
| Navarre | Public | Universidad Pública de Navarra | Pamplona |
| Private | University of Navarre | Pamplona |
| Valencian Community | Public | James I University | Castellón de la Plana |
| Miguel Hernández University of Elche | Elche, Alicante |
| Polytechnic University of Valencia | Valencia |
| University of Alicante | San Vicente del Raspeig, Alicante |
| University of Valencia | Valencia |
| Private | Universidad Católica de Valencia San Vicente Mártir | Valencia |
| Universidad CEU Cardenal Herrera | Moncada, Valencia |
