John Gregory may refer to:
- John Gregory (moralist) (1724–1773), Scottish author
- John Munford Gregory (1804–1884), Governor of Virginia, United States, 1842–1843
- John Gregory (governor), British Governor of the Bahamas, 1854–1857
- John Milton Gregory (1822–1898), first President of the University of Illinois at Urbana-Champaign, United States, 1867–1880
- John Walter Gregory (1864–1932), British geologist
- John Duncan Gregory (1877–1951), British diplomat dismissed for speculation in foreign currencies
- John Gregory (politician) (1878–1955), Canadian member of Parliament
- Colin Gregory (1903–1959), British tennis player, born John Colin Gregory
- John Gregory (football coach) (born 1938), American football coach, current head coach of the Iowa Barnstormers
- John Gregory (footballer) (born 1954), English footballer and coach
- John M. Gregory (businessman), American former CEO of King Pharmaceuticals, Inc.
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