To date there has been no female Governor of Gibraltar, other than in an acting position.
| Picture | Term | Incumbent | Notes |
| 13 July 1713 | Spain cedes Gibraltar to Great Britain in the Treaty of Utrecht |
| 13 July 1713 to 7 August 1713 | Thomas Stanwix, Governor |
 | 7 August 1713 to 20 October 1720 | The Earl of Portmore, Governor |
| 20 October 1720 to 2 February 1727 | Richard Kane, Governor |
| 2 February 1727 to 13 May 1730 | Jasper Clayton, Governor |
| 15 May 1730 to 24 October 1739 | Joseph Sabine, Governor |
| 24 October 1739 to 22 April 1740 | Francis Columbine, Governor |
| 22 April 1740 to 1749 | William Hargrave, Governor |
| 1749 to 31 May 1754 | Humphrey Bland, Governor |
| 31 May 1754 to 12 July 1756 | Thomas Fowke, Governor |
 | 12 July 1756 to 16 April 1757 | The Lord Tyrawley, Governor |
| 16 April 1757 to 28 April 1761 | The Earl of Home, Governor |
| 28 April 1761 to 23 June 1761 | John Toovey, acting Governor |
| 13 June 1761 to 14 June 1761 | John Parslow, acting Governor |
 | 14 June 1761 to January 1776 | Edward Cornwallis, Governor |
| 1765 to 1767 | John Irwin, acting Governor |
| January 1776 to 25 May 1777 | Robert Boyd, acting Governor | 1st Term |
 | 25 May 1777 to 14 June 1787 | George Eliott, Governor |
 | 14 June 1787 to July 1790 | The Lord Heathfield, Governor |
| July 1790 to October 1790 | Sir Robert Boyd, acting Governor |
| October 1790 to 13 May 1794 | Sir Robert Boyd, Governor | 2nd Term |
 | 1794-1795 | Henry Clinton, Governor | Died in London before taking up the post |
| 13 May 1794 to 30 December 1795 | Charles Rainsford, Governor |
 | 30 December 1795 to 25 February 1802 | Charles O'Hara, Governor |
| 25 February 1802 to 10 May 1802 | Charles Barnett, Governor |
 | 24 May 1802 to 23 January 1820 | The Duke of Kent |
| 2 May 1803 to 17 December 1804 | Sir Thomas Trigge, acting Governor | For the Duke of Kent |
 | 17 December 1804 to June 1806 | Henry Edward Fox, acting Governor | For the Duke of Kent |
| June 1806 to November 1806 | James Drummond, acting Governor | 1st Term; for the Duke of Kent |
 | November 1806 to August 1808 | Sir Hew Dalrymple, acting Governor | For the Duke of Kent |
| August 1808 to May 1809 | James Drummond, acting Governor | 2nd Term; for the Duke of Kent |
 | May 1809 to August 1809 | Sir John Cradock, acting Governor | For the Duke of Kent |
| August 1809 to October 1809 | John Smith, acting Governor | For the Duke of Kent |
| October 1809 to November 1809 | Alex McKenzie Fraser, acting Governor | For the Duke of Kent |
| November 1809 to 2 April 1814 | Colin Campbell, acting Governor | For the Duke of Kent |
 | 3 April 1814 to 15 November 1821 | Sir George Don, acting Governor | 1st Term; for the Duke of Kent |
 | 29 January 1820 to 1835 | The Earl of Chatham, Governor |
 | 7 June 1825 to 20 June 1830 | Sir George Don, acting Governor | 2nd Term; for the Earl of Chatham |
| Picture | Term | Incumbent | Notes |
| 1830 | Gibraltar becomes a British Crown colony |
 | 20 June 1830 to 10 May 1831 | Sir George Don, acting Governor | 2nd Term; for the Earl of Chatham |
| 10 May 1831 to 29 May 1835 | Sir William Houstoun, acting Governor | For the Earl of Chatham |
| 28 February 1835 to 11 October 1842 | Sir Alexander Woodford, Governor |
 | 11 October 1842 to 12 December 1848 | Sir Robert Wilson, Governor |
 | 12 December 1848 to 26 July 1855 | Sir Robert Gardiner, Governor |
| 26 July 1855 to 5 May 1859 | Sir James Fergusson, Governor |
| 5 May 1859 to 21 September 1865 | Sir William Codrington, Governor |
| 21 September 1865 to 25 July 1870 | Sir Richard Airey, Governor |
 | 25 July 1870 to 23 June 1876 | Sir William Williams, Governor |
 | 23 June 1876 to 3 January 1883 | The Lord Napier of Magdala, Governor |
| 3 January 1883 to 2 November 1886 | Sir John Miller Adye, Governor |
| 2 November 1886 to 23 August 1890 | Sir Arthur Hardinge, Governor |
| 23 August 1890 to 27 January 1891 | Sir Leicester Smyth, Governor |
| 27 January 1891 to 31 March 1891 | H. R. L. Newdigate, acting Governor |
 | 31 March 1891 to 27 June 1893 | Sir Lothian Nicholson, Governor |
| 27 June 1893 to 7 August 1893 | G.J. Smart, acting Governor |
| 7 August 1893 to 22 May 1900 | Sir Robert Biddulph, Governor |
 | 22 May 1900 to 1 August 1905 | Sir George Stuart White, Governor |
 | 1 August 1905 to 30 July 1910 | Sir Frederick Forestier-Walker, Governor |
 | 30 July 1910 to 11 July 1913 | Sir Archibald Hunter, Governor |
 | 11 July 1913 to 9 July 1918 | Sir Herbert Miles, Governor |
 | 9 July 1918 to 26 May 1923 | Sir Horace Smith-Dorrien, Governor |
 | 26 May 1923 to 13 August 1928 | Sir Charles Monro, Governor |
 | 13 August 1928 to 13 May 1933 | Sir Alexander Godley, Governor |
 | 13 May 1933 to 12 August 1938 | Sir Charles Harington Harington, Governor |
 | 12 August 1938 to 11 July 1939 | Sir Edmund Ironside, Governor |
| 11 July 1939 to 14 May 1941 | Sir Clive Gerard Liddell, Governor |
 | 14 May 1941 to 31 May 1942 | The Viscount Gort, Governor |
 | 31 May 1942 to 14 February 1944 | Sir Noel Mason-Macfarlane, Governor |
| 14 February 1944 to 8 February 1947 | Sir Ralph Eastwood, Governor |
 | 8 February 1947 to 23 April 1952 | Sir Kenneth Anderson, Governor |
| 23 April 1952 to 6 May 1955 | Sir Gordon MacMillan, Governor |
| 6 May 1955 to 16 April 1958 | Sir Harold Redman, Governor |
 | 16 April 1958 to 8 June 1962 | Sir Charles Keightley, Governor |
 | 8 June 1962 to 5 August 1965 | Sir Alfred Ward, Governor |
 | 5 August 1965 to March 1969 | Sir Gerald Lathbury, Governor |
 | March 1969 to 3 October 1973 | Sir Varyl Begg, Governor |
 | 3 October 1973 to 30 May 1978 | Sir John Grandy, Governor |
| 30 May 1978 to 1981 | Sir William Jackson, Governor |