Fixed Dose Procedure Information & Fixed Dose Procedure Links at HealthHaven.com

Fixed Dose Procedure (FDP), proposed in 1984, is a method to assess a substance's acute oral toxicity.^[1] In comparison to older LD₅₀ test developed in 1927, this procedure produces accurate, reproducible results using fewer animals and causing less pain and suffering.^[2] This test is therefore one of the replacements for the LD50 test that is recommended by the Organisation for Economic Co-operation and Development.

FDP uses about 10 to 20 animals to find the dose producing toxicity signs but not death, and from there predicts the lethal dose. LD₅₀ ("lethal dose 50%") uses about 60 to 80 animals to find a dose killing 50% of animals within a given time. FDP sometimes requires retesting using slightly higher or lower doses.

[edit] References

^ Walum E (1998). "Acute oral toxicity". Environ. Health Perspect. 106 Suppl 2: 497–503. doi:10.2307/3433801. PMID 9599698. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1533392&blobtype=pdf.
^ van den Heuvel MJ, Clark DG, Fielder RJ, et al. (1990). "The international validation of a fixed-dose procedure as an alternative to the classical LD₅₀ test". Food Chem. Toxicol. 28 (7): 469–82. doi:10.1016/0278-6915(90)90117-6. PMID 2210519.

[edit] See also

Up-and-down procedure

[edit] Further reading

Stallard, N.; Whitehead, A. (1995). "Reducing animal numbers in the fixed-dose procedure". Human & Experimental Toxicology 14: 315. doi:10.1177/096032719501400401. edit

[0] Walum E (1998). "Acute oral toxicity". Environ. Health Perspect. 106 Suppl 2: 497–503. doi:10.2307/3433801. PMID 9599698. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1533392&blobtype=pdf.

[1] van den Heuvel MJ, Clark DG, Fielder RJ, et al. (1990). "The international validation of a fixed-dose procedure as an alternative to the classical LD₅₀ test". Food Chem. Toxicol. 28 (7): 469–82. doi:10.1016/0278-6915(90)90117-6. PMID 2210519.

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