DUI is a three letter acronym (or initialism) that may stand for:

• Driving under the influence (of drugs such as alcohol)
• Democratic Union for Integration — the largest ethnic Albanian party in the Republic of Macedonia
• Data Use Identifier
• "D.U.I." — a song by punk rock band The Offspring.
• Data Use Institute
• Davis Unified Ignition
• Diving Unlimited International Inc.
• Documento Único de Identidad — all-purpose identification document in El Salvador
• Documento Unico de Importación
• Duke University Improv
• Distinctive unit insignia — a metal heraldic device worn by soldiers in the U.S. Army
• A shortname for the creator god Tuisto of the Celts
• Melvil Dui, Melvil Dewey's considered name following his proposals for English spelling reform

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