Chief O'Hara may refer to several fictional characters, all of which are chiefs of police:

• Chief John O'Hara, a character in Mickey Mouse stories.
• In Batman stories, it may refer to:
  • Chief Miles O'Hara, who appeared in the 1966-1968 Batman television series.
  • Chief Clancy O'Hara, the first victim of the Hangman killer in the Batman: Dark Victory comic book series.

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