Biosphere may refer to:

• Biosphere, the part of a planet's shell where all life occurs
• Biosphere, a 1994 song by In Flames on their Subterranean (EP).
• Biosphere 2, an artificial closed ecological system in Oracle, Arizona
• BIOS-3, the first man made biosphere for sustaining humans in a closed ecology.
• Montreal Biosphère, a geodesic dome in Montreal, Canada
• Biosphere (musician), a Norwegian musician
• biosphere reserve, a type of reserve initiated by UNESCO
• The Biosphere, an important book by Russian and Soviet geologist Vladimir Vernadsky
• A closed ecological system in a lifeless environment, ubiquitous in science fiction.

See also Bio-Dome, a movie based on the idea.

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