BamHI is a restriction enzyme, derived from Bacillus amyloliquefaciens. It has the recognition site (G'GATCC), and leaves a sticky end.^[1] One of the earlier enzymes to be used, it is popular for historical reasons, but also because digestion leaves a GATC overhang compatible with many other enzymes. Persistent issues with this specific enzyme spontaneously failing to generate cloneable fragments necessitate aliquoting prior to use. The phenomenon behind these issues is not understood.

[edit] Recognition site

 G G A T C C C C T A G G 

[edit] References

1. ^ Molecular cell biology. Lodish, Harvey F. 5. ed. : - New York : W. H. Freeman and Co., 2003, 973 s. b ill. ISBN 0-7167-4366-3

[edit] External links

• MeSH Deoxyribonuclease+BamHI

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