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An arterial blood gas (ABG) is a blood test that is performed using blood from an artery. It involves puncturing an artery with a thin needle and syringe and drawing a small volume of blood. The most common puncture site is the radial artery at the wrist, but sometimes the femoral artery in the groin or other sites are used. The blood can also be drawn from an arterial catheter.

The test is used to determine the pH of the blood, the partial pressure of carbon dioxide and oxygen, and the bicarbonate level. Many blood gas analyzers will also report concentrations of lactate, hemoglobin, several electrolytes, oxyhemoglobin, carboxyhemoglobin and methemoglobin. ABG testing is mainly used in pulmonology, to determine gas exchange levels in the blood related to lung function, but has a variety of applications in other areas of medicine. Combinations of disorders can be complex and difficult to interpret, so calculators ^[1], nomograms, and rules of thumb^[2] are commonly used.

Contents 1 Extraction and analysis 2 Reference ranges and interpretation 3 See also 4 References 5 External links

[edit] Extraction and analysis

Arterial blood for blood gas analysis is usually extracted by a phlebotomist, nurse, or respiratory therapist.^[3] Blood is most commonly drawn from the radial artery because it is easily accessible, can be compressed to control bleeding, and has less risk for occlusion. The femoral artery (or less often, the brachial artery) is also used, especially during emergency situations or with children. Blood can also be taken from an arterial catheter already placed in one of these arteries.

The syringe is pre-packaged and contains a small amount of heparin, to prevent coagulation or needs to be heparinised, by drawing up a small amount of heparin and squirting it out again. Once the sample is obtained, care is taken to eliminate visible gas bubbles, as these bubbles can dissolve into the sample and cause inaccurate results. The sealed syringe is taken to a blood gas analyzer. If the sample cannot be immediately analyzed, it is chilled in an ice bath in a glass syringe to slow metabolic processes which can cause inaccuracy. Samples drawn in plastic syringes are not iced and are analyzed within 30 minutes.^[4]

The machine used for analysis aspirates this blood from the syringe and measures the pH and the partial pressures of oxygen and carbon dioxide. The bicarbonate concentration is also calculated. These results are usually available for interpretation within five minutes.

Standard blood tests can also be performed on arterial blood, such as measuring glucose, lactate, hemoglobins, dys-haemoglobins, bilirubin and electrolytes.

[edit] Reference ranges and interpretation

These are typical reference ranges, although various analysers and laboratories may employ different ranges.

Analyte	Range	Interpretation
pH	7.35 - 7.45	The pH or H+ indicates if a patient is acidotic (pH < 7.35; H+ >45) or alkalemic (pH > 7.45; H+ < 35).
H+	35 - 45 nmol/l (nM)	See above.
PaO2	9.3-13.3 kPa or 80-100 mmHg	A low O2 indicates that the patient is not respiring properly, and is hypoxemic. At a PaO2 of less than 60 mm Hg, supplemental oxygen should be administered. At a PaO2 of less than 26 mm Hg, the patient is at risk of death and must be oxygenated immediately.
PaCO2	4.7-6.0 kPa or 35-45 mmHg	The carbon dioxide and partial pressure (PaCO2) indicates a respiratory problem: for a constant metabolic rate, the PaCO2 is determined entirely by ventilation.[5] A high PaCO2 (respiratory acidosis) indicates underventilation, a low PaCO2 (respiratory alkalosis) hyper- or overventilation. PaCO2 levels can also become abnormal when the respiratory system is working to compensate for a metabolic issue so as to normalize the blood pH. An elevated PaCO2 level is desired in some disorders associated with respiratory failure; this is known as permissive hypercapnia.
HCO3-	22–26 mmol/l	The HCO3- ion indicates whether a metabolic problem is present (such as ketoacidosis). A low HCO3- indicates metabolic acidosis, a high HCO3- indicates metabolic alkalosis. HCO3- levels can also become abnormal when the kidneys are working to compensate for a respiratory issue so as to normalize the blood pH.
SBCe	21 to 27 mmol/l	the bicarbonate concentration in the blood at a CO2 of 5.33 kPa, full oxygen saturation and 37 degrees Celsius.[6]
Base excess	-3 to +3 mmol/l	The base excess is used for the assessment of the metabolic component of acid-base disorders, and indicates whether the patient has metabolic acidosis or metabolic alkalosis. A negative base excess indicates that the patient has metabolic acidosis (primary or secondary to respiratory alkalosis). A positive base excess indicates that the patient has metabolic alkalosis (primary or secondary to respiratory acidosis).[7]
HPO42−	0.8 to 1.5 [8] mM
total CO2 (tCO2 (P)c)	25 to 30 mmol/l	This is the total amount of CO2, and is the sum of HCO3- and PCO2 by the formula: tCO2 = [HCO3-] + α*PCO2, where α=0.226 mM/kPa, HCO3- is expressed in millimolar concentration (mM) (mmol/l) and PCO2 is expressed in kPa [9]
total O2 (tO2e)		This is the sum of oxygen solved in plasma and chemically bound to hemoglobin. [10]

Contamination with room air will result in abnormally low carbon dioxide and (generally) normal oxygen levels. Delays in analysis (without chilling) may result in inaccurately low oxygen and high carbon dioxide levels as a result of ongoing cellular respiration.

Lactate level analysis is often featured on blood gas machines in neonatal wards, as infants often have elevated lactic acid.

[edit] See also

• Acid-base homeostasis
• Anion gap
• Mechanical ventilation

[edit] References

[edit] External links

• Alan Grogono's comprehensive guide to acid-base physiology
• An online model of arterial blood gas changes with respiration
• Online arterial blood gas interpreter
• Interactive ABG quiz
• RT Corner (Educational Site for RT's and Nurses) at rtcorner.net

v • d • e Respiratory system, physiology: respiratory physiology Lung volumes VC · FRC · Vt · dead space · CC calculations: respiratory minute volume · FEV1/FVC ratio devices: spirometry · body plethysmography · peak flow meter Airways/ ventilation (V) positive pressure ventilation · breath (inhalation, exhalation) · respiratory rate · respirometer · pulmonary surfactant · compliance · hysteresivity · airway resistance · bronchial hyperresponsiveness · bronchial challenge test · bronchoconstriction/bronchodilation Blood/ perfusion (Q) pulmonary circulation · hypoxic pulmonary vasoconstriction · pulmonary shunt Interactions/ ventilation/perfusion ratio (V/Q) ventilation/perfusion scan · zones of the lung · gas exchange · pulmonary gas pressures · alveolar gas equation  · alveolar-arterial gradient  · hemoglobin · oxygen-haemoglobin dissociation curve (2,3-DPG, Bohr effect, Haldane effect) · carbonic anhydrase (chloride shift) · oxyhemoglobin · respiratory quotient  · arterial blood gas  · diffusion capacity (DLCO) Control of respiration pons (pneumotaxic center, apneustic center) · medulla (dorsal respiratory group, ventral respiratory group) · chemoreceptors (central, peripheral) · pulmonary stretch receptors (Hering-Breuer reflex) Insufficiency high altitude · oxygen toxicity · hypoxia respiratory system navs: anat nose,larynx/lower+thoracic cavity/physio/dev, noncongen/congen/tumors, symptoms+signs/eponymous, proc

v • d • e Urinary system, physiology: renal physiology and acid-base physiology Filtration Renal blood flow · Ultrafiltration · Countercurrent exchange · Filtration fraction Hormones affecting filtration Antidiuretic hormone (ADH) · Aldosterone · Atrial natriuretic peptide Secretion/clearance Pharmacokinetics · Clearance of medications · Urine flow rate Reabsorption Solvent drag · Na+ · Cl- · urea · glucose · oligopeptides · protein Endocrine Renin · Erythropoietin (EPO) · Calcitriol (Active vitamin D) · Prostaglandins Assessing Renal function/ Measures of dialysis Glomerular filtration rate · Creatinine clearance · Renal clearance ratio · Urea reduction ratio · Kt/V · Standardized Kt/V · Hemodialysis product · PAH clearance (Effective renal plasma flow · Extraction ratio) Acid-base physiology Fluid balance · Darrow Yannet diagram · Body water · Interstitial fluid · Extracellular fluid · Intracellular fluid/Cytosol · Plasma · Transcellular fluid · Base excess · Davenport diagram · Anion gap · Arterial blood gas · Winter's formula Buffering/compensation Bicarbonate buffering system · Respiratory compensation · Renal compensation Other Fractional sodium excretion · BUN-to-creatinine ratio · Tubuloglomerular feedback · Natriuresis urinary system navs: anat/physio/dev, noncongen/acid+base/congen/neoplasia, symptoms+signs/eponymous, proc

v • d • e Medical test: Serology, reference range: blood tests Clinical biochemistry Metabolic panel BMP: electrolytes (Na+/K+, Cl-/HCO3-) · renal function, BUN-to-creatinine ratio (BUN/Creatinine) · Glucose · Ca CMP: BMP + protein tests (Human serum albumin, Serum total protein) · liver function tests (ALP, ALT, AST, Bilirubin) derived values: Plasma osmolality · Serum osmolal gap Acid-base homeostasis Arterial blood gas · Base excess · Anion gap · CO2 content Iron tests Transferrin saturation = Serum iron / Total iron-binding capacity Ferritin · Transferrin · Transferrin receptor Blood sugar Glucose test · Glucose tolerance test · Glycemia · Noninvasive glucose · C-peptide · Fructosamine · Random glucose test · Glycosylated hemoglobin Endocrine ACTH stimulation test · Thyroid function tests Cardiac marker Troponin test · CPK-MB test · Glycogen phosphorylase isoenzyme BB Other Beutler test · Blood lipids · Tumor marker Hematology/CBC Clotting vWF: Ristocetin induced platelet agglutination clotting factors: Prothrombin time · Partial thromboplastin time · Thrombin time other/general coagulation: Bleeding time · animal enzyme (Reptilase time, Ecarin clotting time, Dilute Russell's viper venom time) · Thromboelastography fibrinolysis: Euglobulin lysis time · D-dimer Red blood cell indices Hematocrit · Hemoglobin · RBC count ratios: Mean corpuscular hemoglobin · Mean corpuscular hemoglobin concentration · Mean corpuscular volume Fetal hemoglobin: Apt-Downey test · Kleihauer-Betke test · Red blood cell distribution width Reticulocyte index · Haptoglobin Other Blood film · Blood viscosity · Absolute neutrophil count Immunology Infections viral infection: HIV (HIV test, BDNA test) · Epstein-Barr virus (Monospot test) bacterial infection: syphilis (VDRL, Rapid plasma reagin, Wassermann test, FTA-ABS) · rickettsia (Weil-Felix test) · helicobacter (HelicoCARE direct) · streptococcus (Antistreptolysin O titre) protozoan infection: toxoplasmosis (Sabin-Feldman dye test) Inflammation C-reactive protein · Erythrocyte sedimentation rate · MELISA · RAST test see also reference ranges for blood tests